TABLE 3.
Autolysis of exponential, stationary, VBNC, and UV-killed cells and walls.
| Autolysis | % Turbidity loss ina:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Cells
|
Walls
|
|||||||
| Exp | Sta | VBNC | UV killed | Exp | Sta | VBNC | UV killed | |
| Due to active mur-1 | 8 (7) | 9 (6) | 13 (18) | 4 (35) | 28 (7) | 31 (10) | 38 (5) | 6 (33) |
| Due to latent and active mur-1 (trypsin treatment) | 10 (6) | 9 (13) | 35 (8) | 5 (41) | 32 (6) | 30 (12) | 74 (3) | 9 (24) |
Lysis is expressed as percent turbidity loss (coefficient of variation is given in parentheses) in 60 min at 640 nm (cells) or 450 nm (walls).