TABLE 1.
Effect of HAP4 overexpression on carbon flux distributiona
| q | Fluxa
|
|
|---|---|---|
| DL1 | DL1HAP | |
| qglucose | −9.84 | −8.08 |
| qethanol | 14.49 | 10.84 |
| qglycerol | 2.31 | 0.62 |
| qacetate | 0.28 | 0.64 |
| qCO2 | 17.4 | 17.4 |
| qO2 | 3.3 | 6.9 |
a
Determined as described in Materials and Methods; calculated from the linear correlations of metabolite concentration versus dry biomass formed during 6 h of exponential growth in a fermentor-batch cultivation, amplified by the specific growth rate. Data are presented as the average of calculations based on duplicate metabolite and biomass determinations from two independent experiments. Data varied by less than 5%.