Figure 4.
The expression of ER-stress-related genes and proteins in TEB-treated MAC-T cells. (A) mRNA expression levels of CHOP, Bip/Grp78, and ATF4 in MAC-T cells by qPCR after 24 h of TEB treatment. (B) Immunoblots for detecting Bip/Grp78, ERO1-Lα, PDI, and β-actin in response to the exposure to different concentrations of TEB. (C) The graphs present the results of densitometric analysis. Each protein band was normalized using β-actin. Data represent the mean ± SD of three independent experiments (n = 3, * p < 0.05, and ** p < 0.001 compared to the controls).