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. 2023 Mar 29;11(4):755. doi: 10.3390/vaccines11040755

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antibody-dependent enhancement (ADE) of ZV1 glycovariants for DENV and ZIKV infection. Serial dilutions of ZV1 glycovariants were mixed with DENV-2 (A) or ZIKV (B) and then incubated with FcγRIIa⁺ K562 cells. After incubation, cells were fixed, permeabilized, and stained with an anti-flavivirus E antibody and then analyzed by flow cytometry to identify DENV-2 or ZIKV-infected cells. Results (Mean ± SD) from at least two independent experiments with technical triplicates for each sample are presented.