TABLE 2.
Effect of DGP and related solutes (at 100 mM concentration) on rabbit muscle LDH and baker's yeast ADH thermostability
| Solute | % Recovered activitya for:
|
|||
|---|---|---|---|---|
| LDH at:
|
ADH at 50°C | |||
| 50°C | 55°C | 60°C | ||
| No additions | 31 ± 3 | 8 ± 2 | 2 ± 1 | 33 ± 3 |
| Glycerolb | 42 ± 4 | 8 ± 2 | 1 ± 1 | 31 ± 1 |
| KPi | 83 ± 5 | 29 ± 6 | 12 ± 3 | 85 ± 11 |
| KPi + glycerolb | 81 ± 4 | 31 ± 3 | 13 ± 2 | 88 ± 9 |
| DGP | 85 ± 8 | 35 ± 6 | 15 ± 2 | 88 ± 5 |
a
Mean values of at least three independent experiments. Values were obtained after incubations of 10 min at the indicated temperatures.
b
The concentration of glycerol was 200 mM to mimic the composition of DGP.