Figure 1.

Generation of precision-cut lung slice (PCLS). Lung tissues are removed from dogs, followed by inflation of 1.5% low-melting agarose through the airways of single lung lobes and solidification on ice for 30 min (A). Lung lobes are further cut into 1 cm thick slices and cylindric pieces containing a cross-sectioned bronchus are generated (B). These are inserted into a tissue slicer (C), which produces a uniform PCLS of 250 µm thickness (D). Hematoxylin-eosin (HE)-stained PCLS section shows retained pulmonary tissue architecture (E).