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. 2023 Mar 24;15(4):834. doi: 10.3390/v15040834

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of canine distemper virus (CDV) in PCLSs by immunofluorescence (A–C) and transmission electron microscopy (D). Immunofluorescence double-labelling detection of CDV antigen (green), cytokeratin (red, A), or Iba-1 (red, B) revealed CDV infection of epithelial cells (white arrows, A), peribronchial histiocytes (white arrows, B), and alveolar macrophages (white arrow, C) at day 6 postinfection. Nuclear counterstaining: bizbenzimide (blue). Scale bars: 50 µm. Intranuclear inclusions of CDV nucleoprotein filaments at day 3 postinfection (D, arrow). Scale bar: 500 nm.