Table 1:
CryoEM data collection, model refinement and validation
| Data Collection | |
|
| |
| Voltage (kV) | 300 |
| Magnification | 96,000× |
| Total electron dose (e−/Å2) | 56.6 |
| Defocus range (μm) | −0.7 to −2.0 |
| Calibrated pixel size (Å) | 0.852 |
| Micrographs collected | 4343 |
|
| |
| Data Processing | |
|
| |
| Extracted particles | 2,154,997 |
| Particles used for final reconstruction | 212,196 |
| Final map resolution (Å, 0.143 FSC) | 2.90 |
| Map resolution range (Å) | 2.5 – 3.2 |
| Map sharpening B factor (Å2) | 103.2 |
|
| |
| Model Content | |
|
| |
| Initial models used (PDB code) | 6WPW (GCGR/Gs/Nb35) |
| Total number of atoms | 8,834 |
| No. of protein residues | 1,112 |
| No. of ligands | 0 |
|
| |
| Model Validation | |
|
| |
| CC map vs. model (%) | 77 |
| RMSD | |
| Bond lengths (Å) / Bond angles (°) | 0.002 / 0.598 |
| Ramachandran plot statistics | |
| Favored (%) | 97.4 |
| Allowed (%) | 2.6 |
| Outliers (%) | 0.0 |
| Rotamer outliers (%) | 0 |
| C-beta deviations | 0 |
| Clash score | 3.83 |