TABLE 2.

Sensitization of gram-negative bacteria to lytic agents

Strain and lytic substance	Concn	Relative turbidity (%) at 4 mina
		Control	Lactic acid (10 mM, pH 3.6)	HCl (pH 3.6)	KCN (1 mM, pH 7.2)
E. coli O157:H7
Lysozyme	10 μg/ml	101 ± 2	99 ± 1	100 ± 1	100 ± 1
Triton X-100	0.1%	104 ± 1	102 ± 1	104 ± 1	103 ± 2
Triton X-100	1%	95 ± 1	92 ± 1	95 ± 1	95 ± 1
SDS	0.1%	102 ± 1	52 ± 11	102 ± 1	97 ± 2
SDS	1%	97 ± 1	47 ± 7	95 ± 1	83 ± 3
P. aeruginosa
Lysozyme	10 μg/ml	99 ± 1	23 ± 1	53 ± 26	101 ± 1
Triton X-100	0.1%	102 ± 3	80 ± 1	55 ± 20	104 ± 1
Triton X-100	1%	98 ± 1	69 ± 1	45 ± 19	92 ± 1
SDS	0.1%	104 ± 2	18 ± 1	17 ± 1	101 ± 1
SDS	1%	101 ± 1	ND	ND	94 ± 1
Salmonella serovar Typhimurium
Lysozyme	10 μg/ml	101 ± 1	102 ± 2	100 ± 1	102 ± 1
Triton X-100	0.1%	104 ± 3	101 ± 4	103 ± 1	104 ± 1
Triton X-100	1%	97 ± 1	91 ± 2	92 ± 2	94 ± 5
SDS	0.1%	102 ± 3	60 ± 10	94 ± 2	98 ± 1
SDS	1%	97 ± 2	53 ± 10	85 ± 3	77 ± 11

^aND, not determined.