TABLE 3.
Liberation of fatty acid-containing material from serovar Typhimurium SL696 by EDTA, lactic acid, and HCl
| Fatty acid | Amt (μg) of fatty acid in 8.6 ml of cell-free supernatant after treatment with:
|
|||
|---|---|---|---|---|
| None (control) | EDTA (1 mM) | Lactic acid (5 mM, pH 3.5) | HCl (pH 3.5) | |
| C12:0a | 1.4 | 2.4 | 2.8 | 2.0 |
| C14:0a | 0.9 | 4.1 | 4.7 | 2.6 |
| C14:0(3-OH)a | 0.8 | 6.1 | 8.6 | 4.6 |
| C16:0 | 3.4 | 6.8 | 10.7 | 7.2 |
| C16:1 | 2.3 | 5.5 | 8.6 | 6.0 |
| C18:1 | 2.5 | 3.1 | 5.5 | 3.1 |
| Total | 11.3 | 28.0 | 40.9 | 25.5 |
a
The percentages of LPS-specific fatty acids for the four treatment groups in columns 2 through 5 are 27, 45, 39, and 36%, respectively.