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Patch-clamp recordings of whole-cell current responses. (A) No inward current was recorded after the application of 3 mM L-Glu + 10 μM glycine (Gly) to undifferentiated SH-SY5Y cells, or (B) differentiated SH-SY5Y cells. (C) A positive control current response was achieved from differentiated TE671 cells exposed to brief applications of 3 mM L-Glu + 10 μM Gly using whole-cell patch-clamp recordings at a holding potential of −50 mV (n = 20).
