Table 2.
Phytocompound | Test | Extract | |||||
---|---|---|---|---|---|---|---|
Leaf | Stem | ||||||
H | C | M | H | C | M | ||
Alkaloids | Wagner’s test | + | + | ++ | + | + | ++ |
Mayer’s test | + | + | + | + | + | + | |
Flavonoids | Lead acetate | ++ | ++ | ++ | ++ | ++ | ++ |
Alkaline reagent test | + | + | + | + | + | + | |
Acid hydrolysis test | + | + | + | + | + | + | |
Phenolic compounds | Ferric chloride test | + | + | + | + | + | + |
Lead acetate test | + | + | + | + | + | + | |
Polyphenols | Ferric chloride test | + | + | + | + | + | + |
Terpenoids | Salkowski’s test | + | + | + | + | + | + |
Liebermann–Burchard test | + | + | + | + | + | + | |
Tannins | Ferric chloride test | + | + | + | + | + | + |
Lead acetate test | + | + | + | + | + | + | |
Gelatin test | + | + | + | + | + | + | |
Coumarin | Sodium hydroxide test | + | + | + | + | + | + |
Saponin | Foam test | + | + | + | + | + | + |
Olive oil test | – | – | + | – | – | + | |
Phytosterols | Salkowski’s test | + | + | + | + | + | + |
Liebermann–Burchard test | + | + | + | + | + | + | |
Gums and mucilage |
Precipitation test | + | – | – | + | – | – |
Ruthenium red test | + | + | + | + | + | + | |
Resins | Acetone test | – | – | – | – | – | – |
Glycosides | Keller–Killani test | – | – | – | – | – | – |
Carbohydrates | Molisch’s test | + | + | + | + | + | + |
Fehling’s test | + | + | + | + | + | + | |
Amino acids and proteins | Ninhydrin test | + | + | + | + | + | + |
Biuret test | + | + | + | + | + | + | |
Lipids and fixed oils | Spot test | – | – | ++ | – | – | ++ |
Note: (++) = intense positive reaction; (+) = positive reaction (–) = negative reaction; H = hexane; C = chloroform; and M = methanol.