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. 2023 Apr 11;11(4):827. doi: 10.3390/vaccines11040827

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Percentage of IgG1 in Spike protein-specific immunoglobulins. Spike-specific immunoglobulins (Spike) were adsorbed by plate-bound recombinant trimeric Spike proteins and analyzed by LC-MS and quantified by XIC AUC. Protein A/G-adsorbed total serum immunoglobulins (Total) were also analyzed. Note that the IgG3 and IgG4 subclasses of rhesus monkey were summarized together due to identical characteristic peptide fragments after trypsin digestion. “*” means significant difference (p < 0.05); “ns” means no significant difference.