FIG. 1.

Spectra of in situ kinetics of [2-¹³C]pyruvate metabolism by resting cells of P. freudenreichii subsp. shermanii CIP 103027. [2-¹³C]pyruvate (360 μmol, corresponding to a final concentration of 124 mM in the NMR tube) was added at time zero to a cell suspension (1 g [wet weight] in 2 ml of sterile saline water) at 24°C (297 K), and proton-decoupled ¹³C NMR spectra were collected every 104 s (64 scans). Chemical shifts were referred to an HMPA capillary. Only five spectra, selected from 33 experiments, are presented. The intensities of the stackplot on the right were multiplied by 0.5.