Fig. 1.

Design and encapsulation of mRNA-A-LNP and mRNA-B-LNP. a The mRNA construct expresses the MPXV-specific antigen A29L, A35R, M1R, and B6R. b The MPXV-specific antigen A29L, A35R, M1R and B6R was expressed by mRNA in HEK293T cells. Cells were transfected with four mRNAs (1 μg/mL) each from mRNA-A-mix and mRNA-B-mix for 20 h using Lipofectamine 3000 transfection reagent. c Preparation mechanism of mRNA-A-LNP and mRNA-B-LNP. Briefly, the four mRNAs were mixed in an acidic aqueous solution, then injected with organic phase lipids, and the mixture was extruded through a microfluidic chip. d The physicochemical parameters of mRNA-A-LNP and mRNA-B-LNP. Data are shown as mean ± SEM. e A representative TEM image presented the morphology of mRNA-A-LNP and mRNA-B-LNP. Scale bar = 200 nm