Table 1.

Zeta Potential, polydispersity and diameter of formulated liposomes at different molar ratios. Measurements were performed in triplicate with cationic liposomes alone or encapsulating 1 μg of RNA replicons encoding GFP with increasing concentrations of liposomes. Unstable ranges of measurements are marked with *.

Formulation	Liposomes	Liposomes + RNA Replicon-Encoding GFP #
		2 nM	4 nM	8 nM	16 nM	32 nM
Zeta Potential (mV) Average ± SD	15.15 ± 1.18	−6.01 ± 0.48	−9.57 ± 0.07	−22.95 ± 1.55	4.4 ± 0.05	8.9 ± 0.2
Polydispersity index Average ± SD	0.225 ± 0.02	0.262 ± 0.01	0.212 ± 0.02	0.221 ± 0.01	*	*
Diameter (nm) Average ± SD	*	*	43.17 ± 1.62	89.94 ± 0.95	155.5 ± 2.05	*

^# All measurements were carried out in triplicate with cationic lipid liposomes alone or with incorporation of the same amount (1 μg) of RNA replicons. * Unstable range of measurements.