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. 2023 Apr 19;24(8):7528. doi: 10.3390/ijms24087528

Figure 3.

Figure 3

JI017 activated autophagy by suppressing the PI3K-AKT-mTOR pathway in H460 and H1299 cells. H460 and H1299 cells were treated with JI017 (50, 100, and 150 μg/mL) for 24 h. (A,B) Whole cell lysates were analyzed by Western blotting. (C) H460 and H1299 cells were treated with JI017 (150 ng/mL) for different times (4, 8, 12, and 24 h). (D) H460 and H1299 cells transfected with the pEGFP-LC3 vector were treated with JI017 (150 μg/mL) for 8 h. The Fluorescence microscopy analysis confirmed positive staining of LC3B puncta. LC3B puncta are indicated by white arrows. Scale bar = 20 µm.