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. 2023 Apr 19;24(8):7528. doi: 10.3390/ijms24087528

Figure 4.

Figure 4

JI017 induced apoptosis through an autophagy pathway and ROS generation. (A) H460 and H1299 cells were treated with 3MA (2 mM) or chloroquine (100 μM) combined with JI017 (150 μg/mL). 3MA, chloroquine, or bafilomycin treatment was followed by treatment with JI017. (B) Cell viability was measured using MTS assay. (C) H460 and H1299 cells were treated with NAC (15 mM) and JI017 (150 μg/mL). Whole cell lysates were analyzed by Western blotting with anti-LC3 and anti-GAPDH antibodies. Data are presented as the mean ± SEM. *** p < 0.001 compared to untreated cells.