FIG. 3.

Substrate specificity of PPT. Reaction mixtures contained different nucleoside monophosphates (AMP, 2′-dAMP, GMP, CMP, UMP, and IMP) and were incubated with the 50% AS cut (0.23 mg of protein per ml; see Materials and Methods). The formation of the corresponding nucleoside di- and triphosphates was monitored by HPLC.