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HK-catalyzed formation of G6P in reaction mixtures with ATP supplied from 5 mM AMP and polyPn = 35 using AdK with different amounts of PPT protein. PPT concentrations, represented as total protein from a 40 to 60% AS cut, are 1.36 (■), 0.68 (⧫), 0.34 (●), and 0.136 (▴) mg/ml. Control reaction mixtures contained 5 mM ATP (dashed lines) with (○) and without (□) AdK.
