TABLE 1.

Expression of polyP:AMP phosphotransferase in crude cell extracts prepared from A. johnsonii sp. 210A grown on different media

Growth condition	Cultivation time (h)	Growth rate (h−1)	Sp act (nmol/min/mg)a
Minimal mediab
Butyrate	23	0.20	68
Crotonate	23	0.17	70
Acetate	18	0.30	64
dl-Lactate	18	0.33	70
Complex media
LB	16.5	NDd	74
LB + KH2PO4 at 0.8 g/liter	16.5	ND	73
LB + dl-lactatec	19	0.40	63
LB + dl-lactatec	31	0.40	61
LB + KH2PO4 + dl-lactatec	25	ND	66

^aReported are averages of duplicate determinations using the spectrophotometric assay. Standard deviations were less than 10%. 

^bDefined media (see Materials and Methods) containing 20 mM carbon source in 200-ml volumes. 

^cMedia contained 40 mM dl-lactate. 

^dND, not determined.