TABLE 2.
AS (% saturation) | Protein concn (mg/ml) | Relative sp act (RLU/mg of protein)a | Relative activity recovery (%) |
---|---|---|---|
0b | 8.6 | 1,620 | 100 |
30 | 2.1 | 37 | 2.2 |
40 | 6.5 | 146 | 9.0 |
50 | 3.5 | 3,124 | 193 |
60 | 2.6 | 139 | 8.5 |
70 | 1.3 | 2 | 0.1 |
PPT activity was measured using assay 2 (see Materials and Methods). The relative specific activity reported represents 10−2 times the actual RLU magnitude measured at 30 min.
100,000 × g HSS.