Figure 6.

Dose-finding experiment in the mouse mastitis model of S. aureus infection. S. aureus ATCC 29740 was inoculated IMAM, at a dose of 100 CFU in a volume of 100 µL, in the 2 fourth glands in lactating CD-1 mice separated from pups 1 h prior to infection. Different doses of StaphLyse™ (in a volume of 100 µL) were administered as a single IMAM dose 4 h after infection. Mammary glands were harvested 18 h after infection, and bacterial loads into the glands were determined (CFU per gram of gland). Each group included 4 mice, with each mouse providing up to 2 glands. Each dot on the graph represents the bacterial load for each gland. The horizontal bars within the boxes indicate the median value for each group. The whiskers show the minimum and maximum values. The results were compared with the results of the placebo control group, which received PBS. Statistical significance was determined using the Kruskal–Wallis comparison test followed by Dunn’s multiple-comparison test (ns: not significant, ** p ˂ 0.01, *** p ˂ 0.001).