Figure 7.

Efficacy of single and repeated doses of StaphLyse™ and effect of route of administration in the S. aureus mouse mastitis model. StaphLyse™ was administered via the IMAM and/or IV routes, once or twice at 4 and/or 8 h after IMAM S. aureus infection in lactating CD-1 mice separated from pups 1 h prior to infection. Each dose of StaphLyse™ contains 10⁸ PFU in a 100 µL volume. Each group contained 4 mice, with each mouse providing 2 glands. Glands were harvested 18 h after infection. The bacterial load for each gland is represented by a dot on the graph. The horizontal bars within the boxes indicate the median value for each group. The whiskers show the minimum and maximum values. Statistical significance was determined using the Kruskal–Wallis comparison test followed by Dunn’s multiple-comparison test (ns: not significant, * p = 0.02, **** p ˂ 0.0001).