Skip to main content
. 2023 Mar 24;15(4):830. doi: 10.3390/v15040830

Figure 2.

Figure 2

Alphavirus production shows differential sensitivity to IFN-induced endogenous ZAP in 293T cells. Following treatment with 5 U/mL IFN-β for 24 h, ZAP KO and WT 293T were infected with RRV (A), SINV (B), ONNV (C), and the CHIKV vaccine strain (181/clone 25) (D) at MOI = 0.1 PFU/cell. Media overlaying the cells were harvested at 0, 8, 24, and 48 h.p.i., and the viral titer was determined by infection of BHK-21 cells in standard plaque assays. Mean values from four biological replicates across two independent experiments are plotted, and error bars represent the SD. Asterisks indicate statistically significant differences (two-way ANOVA and Bonferroni posttest: **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).