Figure 8.

ZAPS and ZAPL binding to CpG-correlated window 1 is CpG-dependent. Lysates of ZAP KO 293T cells with induced expression of RFP-ZAPS (A) or RFP-ZAPL (B) were incubated with biotinylated RRV CGlo, SINV CGlo, ONNV CGhi, or CHIKV CGhi window 1 mutant RNA. Following streptavidin-mediated pull-down of biotinylated RNA, ZAP associated with each RNA and in whole cell lysate (input) was assayed by immunoblot. Expected sizes: RFP-ZAPS = 106 kDa, RFP-ZAPL = 130 kDa. Data are representative of results from three independent experiments.