TABLE 1.
Kinetic properties of the laccases on HBT, VA, and NHA at pH 5.5a
| Laccase | Substrate | Mean ± SD
|
Optimal pH | |
|---|---|---|---|---|
| Km (mM) | kcat (min−1) | |||
| TvL | HBT | 15 ± 3 | 84 ± 6 | 5–6 |
| VA | 5 ± 1 | 260 ± 20 | 6 | |
| NHA | 0.9 ± 0.3 | 470 ± 60 | 5 | |
| BcL | HBT | 12 ± 4 | 10 ± 1 | 6 |
| VA | 11 ± 1 | 40 ± 2 | 5 | |
| NHA | 1.5 ± 0.5 | 160 ± 20 | 5 | |
| PcL | HBT | 29 ± 7 | 22 ± 2 | 6 |
| VA | 9 ± 1 | 370 ± 20 | 4–5 | |
| NHA | 2.2 ± 0.6 | 1,500 ± 200 | 4 | |
| RsL | HBT | 10 ± 2 | 0.57 ± 0.02 | 5 |
| VA | 2.7 ± 0.4 | 46 ± 2 | 4 | |
| NHA | 2.0 ± 0.5 | 150 ± 10 | 7 | |
| CcL | HBT | 7 ± 2 | 0.45 ± 0.05 | 6 |
| VA | 5 ± 1 | 10 ± 1 | 5 | |
| NHA | 3 ± 2 | 17 ± 5 | 6 | |
| StL | HBT | 31 ± 16 | 1.3 ± 0.3 | 5 |
| VA | 0.35 ± 0.08 | 3.2 ± 0.3 | 6 | |
| NHA | 12 ± 3 | 6 ± 1 | 7 | |
| MtL | HBT | 10 ± 8 | 0.12 ± 0.05 | 6 |
| VA | 18 ± 2 | 27 ± 1 | 4 | |
| NHA | ≥20 | ≥36 | 7 | |
a
The Km and kcat data for TvL-, BcL-, PcL-, and MtL-catalyzed HBT and VA oxidations are taken from reference 22.