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. 2023 Apr 20;12(8):1704. doi: 10.3390/plants12081704

Figure 2.

Figure 2

STRP overexpression and protein levels in transgenic lines. STRP overexpression and protein levels were assessed on wt, STRP–overexpressing 1 (OE1), OE2, and OE3 seedlings grown for two weeks on MS medium. (A) STRP overexpression was determined on total RNA extracted from A. thaliana, retrotranscribed, and analyzed by RT-qPCR. Error bars are s.e.m. of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, by Student’s t-test. STRP levels in wt plants and the three OE lines were determined by Western blot using 20 μg of whole cellular extract. STRP was detected by incubating the membrane with anti-YFP (B) and anti-STRP (C) antibodies. Actin was used as a loading control.