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. 2000 May;66(5):2071–2078. doi: 10.1128/aem.66.5.2071-2078.2000

TABLE 2.

Sensitivities of several sets of primers designed for the detection of E. amylovora by various PCR proceduresa

Sampleb Lowest positive dilution (CFU/ml) detected by PCR procedure
Bereswill et al. (2)c McManus and Jones (27)c Maes et al. (23)c Guilford et al. (11)c PEANT1-PEANT2d (this work) S. nestede (27)c N1Tf (this work)
PMV 6089 + water 7 × 102 7 × 101 7 × 102 7 × 103 7 × 101 7 × 10−1 7 × 10−1
PMV 6089 + pear 5 × 102 5 × 103 5 × 103 5 × 104 5 × 102 5 5
PMV 6089 + apple 5 × 103 5 × 102 5 × 102 5 × 104 5 × 102 5 5
PMV 6089 + Pyracantha 5 × 103 5 × 102 5 × 103 5 × 104 5 × 102 5 5
a

Samples of plant extracts were analyzed following DNA extraction. 

b

Serial dilutions of E. amylovora strain PMV 6089 in water and in different plant extracts. 

c

Reference of the primers employed on each analysis. 

d

PEANT1-PEANT2, internal primers designed for the nested-PCR method in one tube. 

e

S. nested, standard nested PCR (two tubes). 

f

N1T, nested PCR in a single closed tube.