TABLE 2.
Sampleb | Lowest positive dilution (CFU/ml) detected by PCR procedure
|
||||||
---|---|---|---|---|---|---|---|
Bereswill et al. (2)c | McManus and Jones (27)c | Maes et al. (23)c | Guilford et al. (11)c | PEANT1-PEANT2d (this work) | S. nestede (27)c | N1Tf (this work) | |
PMV 6089 + water | 7 × 102 | 7 × 101 | 7 × 102 | 7 × 103 | 7 × 101 | 7 × 10−1 | 7 × 10−1 |
PMV 6089 + pear | 5 × 102 | 5 × 103 | 5 × 103 | 5 × 104 | 5 × 102 | 5 | 5 |
PMV 6089 + apple | 5 × 103 | 5 × 102 | 5 × 102 | 5 × 104 | 5 × 102 | 5 | 5 |
PMV 6089 + Pyracantha | 5 × 103 | 5 × 102 | 5 × 103 | 5 × 104 | 5 × 102 | 5 | 5 |
Samples of plant extracts were analyzed following DNA extraction.
Serial dilutions of E. amylovora strain PMV 6089 in water and in different plant extracts.
Reference of the primers employed on each analysis.
PEANT1-PEANT2, internal primers designed for the nested-PCR method in one tube.
S. nested, standard nested PCR (two tubes).
N1T, nested PCR in a single closed tube.