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. 2000 May;66(5):2071–2078. doi: 10.1128/aem.66.5.2071-2078.2000

TABLE 3.

Detection of E. amylovora in naturally infected plant material by various PCR procedures

Referencea (host) No. of samples No. of positive samples detected with primer set
Bereswill et al. (2)b McManus and Jones (27)b Maes et al. (23)b S. nestedc (27)b N1Td (this work)
1899-f (Crataegus azarolus) 1 0 0 0 0 1
1913-b (Crataegus monogyna) 2 0 0 0 0 1
1899-g (Crataegus sp.) 2 1 2 2 2 2
1913-c (Cotoneaster dammeri) 2 0 1 1 1 2
1899-e (Cydonia sp.) 6 0 0 0 0 2
1899-d (Eryobotria japonica) 1 0 0 0 1 1
1899-h (Malus domestica) 4 1 1 1 1 1
1961-b (Malus domestica) 3 3 3 3 3 3
1913-a (Pyracantha sp.) 2 0 1 0 0 1
1952-b (Pyracantha sp.) 15 9 9 8 13 13
1961-c (Pyracantha sp.) 5 4 3 3 5 5
1966-b (Pyracantha sp.) 7 6 6 6 6 6
2112-b (Pyracantha sp.) 5 4 3 4 4 4
1892-b (Pyrus communis) 11 6 9 6 10 10
1961-d (Pyrus communis) 5 4 3 4 4 4
1961-e (Pyrus communis) 5 4 2 4 4 4
1961-f (Pyrus communis) 1 0 0 0 1 1
1961-g (Pyrus communis) 5 1 2 2 3 3
1961-h (Pyrus pyrifolia) 1 1 1 1 1 1
 Total no. of samples 83 44 46 45 59 65
a

Samples with different reference numbers (not including suffix letter) have different origins. 

b

Reference of the primers employed on each analysis. 

c

S. nested, standard nested PCR (two tubes). 

d

N1T, nested PCR in a single closed tube.