TABLE 4.
Detection of E. amylovora in symptomless plant material by various PCR procedures
| Referencea (host) | No. of samples | No. of positive samples detected with each set
|
||||
|---|---|---|---|---|---|---|
| Bereswill et al. (2)b | McManus and Jones (27)b | Maes et al. (23)b | S. nestedc (27)b | N1Td (this work) | ||
| 1898-a (Cotoneaster sp.) | 1 | 1 | 1 | 1 | 1 | 1 |
| 1910-a (Crataegus sp.) | 6 | 0 | 1 | 0 | 1 | 4 |
| 1899-c (Cydonia sp.) | 2 | 0 | 0 | 1 | 0 | 1 |
| 1899-b (Eriobotrya japonica) | 1 | 0 | 0 | 0 | 1 | 1 |
| 1898-b (Malus sp.) | 2 | 1 | 1 | 1 | 1 | 1 |
| 1961-a (Malus sp.) | 8 | 7 | 7 | 7 | 7 | 7 |
| 1898-c (Pyracantha sp.) | 1 | 1 | 1 | 1 | 1 | 1 |
| 1952-a (Pyracantha sp.) | 4 | 3 | 3 | 1 | 4 | 4 |
| 1966-a (Pyracantha sp.) | 5 | 5 | 5 | 2 | 5 | 5 |
| 1967 (Pyracantha sp.) | 2 | 1 | 2 | 2 | 2 | 2 |
| 2112-a (Pyracantha sp.) | 3 | 3 | 3 | 3 | 3 | 3 |
| 2161 (Pyracantha sp.) | 30 | 1 | 1 | 0 | 1 | 1 |
| 1895 (Pyrus communis) | 14 | 0 | 1 | 1 | 2 | 3 |
| 1899-a (Pyrus communis) | 1 | 0 | 1 | 0 | 0 | 1 |
| 1886 (Pyrus sp.) | 4 | 4 | 4 | 4 | 4 | 4 |
| 1892-a (Pyrus sp.) | 1 | 0 | 0 | 0 | 1 | 1 |
| 1910-b (Pyrus sp.) | 9 | 0 | 1 | 0 | 3 | 7 |
| 2083 (Pyrus sp.) | 40 | 0 | 1 | 1 | 1 | 1 |
| 2116 (Pyrus sp.) | 18 | 0 | 8 | 10 | 12 | 13 |
| 2181 (Pyrus sp.) | 99 | 0 | 1 | 1 | 1 | 1 |
| Total no. of samples | 251 | 27 | 42 | 36 | 51 | 62 |
a
Samples with different reference numbers (not including suffix letter) have different origins.
b
Reference of the primers employed on each analysis.
c
S. nested, standard nested PCR (two tubes).
d
N1T, nested PCR in a single closed tube.