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. 2000 May;66(5):2071–2078. doi: 10.1128/aem.66.5.2071-2078.2000

TABLE 5.

Confirmation by other techniques of some samples positive by nested PCR in one single closed tube

Species Reference Result byd:
Other PCRa E-ELISAb Isolationc
Cotoneaster sp. 1898-2 + +
Crataegus sp. 1910-11 +
Cydonia sp. 1899-5 +
Eriobotrya japonica 1899-20 +
Malus domestica 1898-11 +
1961-7 +
1961-26 + +
1961-27 +
1961-29 +
1961-30 + +
1961-31 + + +
1961-32 + + +
Pyracantha sp. 1898-7 +
1952-6 +
1952-12 +
1952-16 + +
1952-19 + + +
1966-1 + +
1966-5 +
1966-7 +
1966-8 +
1966-11 + + +
1967-1 +
1967-2 +
2112-1 + + +
2112-4 + + +
2112-10 + + +
2161-26 +
Pyrus sp. 1886-2 + + +
1886-3 + +
1886-4 + +
1886-5 + +
1892-9 +
1895-3 +
1895-10 +
1899-1 +
1910-3 +
1910-6 +
1910-11 +
2083-39 +
2116-2 +
2116-3 +
2116-5 +
2116-6 +
2116-7 +
2116-8 +
2116-9 +
2116-11 +
2116-13 +
2116-15 + +
2116-16 + +
2116-18 +
2116-19 +
a

Standard and two-tube nested PCR using primers described by Bereswill et al. (2), McManus and Jones (27), and Maes et al. (23). 

b

Enrichment-ELISA-DASI using specific monoclonal antibodies (Gorris et al. [9]). 

c

Isolation on common and selective media (18, 15). 

d

+, positive; −, negative.