FIG. 4.

Effect of culturing Vibrio harveyi in the presence of 200 nM HBHL and 10% high-density culture supernatant on the appearance of toxin T1 in low-density (OD₆₀₀ = 0.1) culture supernatants as determined by native PAGE. The gel shows supernatant extract from untreated (OD₆₀₀ = 0.1) culture (lane 1), supernatant extract from HBHL-treated (OD₆₀₀ = 0.1) culture (lane 2), supernatant extract from 10% high-density supernatant-treated (OD₆₀₀ = 0.1) culture (lane 3), and a 10-fold dilution of high-density supernatant extract from untreated (OD₆₀₀ = 0.4) culture (lane 4). The arrowhead indicates toxin T1.