TABLE 2.
Survival rates of CBA mice and juvenile P. monodon specimens challenged with concentrated cell supernatant extracts prepared from cultures of V. harveyi 47666-1 and treated postextraction with 100 μM halogenated furanone
| Subjects | Untreated extracta
|
Furanone-treated extractb
|
||
|---|---|---|---|---|
| No. surviving/no. injected (% surviving) | Dose (μg/g) | No. surviving/no. injected (% surviving) | Dose (μg/g) | |
| CBA mice | 0/3 (0) | 10.6 | 0/3 (0) | 10.4 |
| 1/3 (33) | 6.1 | 0/3 (0) | 6.0 | |
| 1/3 (33) | 4.2 | 1/3 (33) | 4.2 | |
| 0/3 (0) | 2.9 | 1/3 (33) | 3.1 | |
| P. monodon specimens | 2/9 (22) | 13.9 | 0/7 (0) | 14 |
| 3/9 (33) | 6.1 | 2/8 (25) | 7.4 | |
| 5/9 (56) | 5.6 | 5/8 (63) | 5.5 | |
| 4/7 (57) | 4.1 | 6/7 (86) | 4.5 | |
| 7/7 (100) | 3.5 | 6/7 (86) | 3.4 | |
a
Total protein concentration = 4.0 μg/ml.
b
Total protein concentration = 3.8 μg/ml.