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. 2023 Apr 17;11(4):856. doi: 10.3390/vaccines11040856

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The number of PP19128R-specific IFN-γ⁺ T lymphocytes. The purified PP19128R vaccine (experiment group), AIM (negative control), and our previously designed vaccine HP13138PB [50] (positive control) were used to stimulate PBMCs collected from HCs (A), ATB patients (B), and LTBI individuals (C) in vitro. The number of antigen-specific IFN-γ⁺ T lymphocytes was detected by ELISpot. SFCs: the number of spot-forming cells; HCs: healthy controls; ATB: active tuberculosis; LTBI: latent tuberculosis infection.