Table 1.
The immunodominant HTL, CTL, and B-cell epitopes of the PP19128R vaccine.
| Antigen | Allele | Peptide | Adjusted Rank | Antigenicity Score | IFN-γ Score | Immunogenicity Score | ABC Score | Allergenicity * | Toxin Pred | |
|---|---|---|---|---|---|---|---|---|---|---|
| HTL | ||||||||||
| Rv1736c | HLA-DQA1*02:01, DQB1*05:02 | GAAFSWYTYSPTRVR | 0.14 | 0.9205 | 0.43799388 | NA | NA | NO | NO | NT |
| HLA-DQA1*05:01, DQB1*02:01 | DFLPVVLEFAATVDPEA | 0.31 | 1.0044 | 0.60486444 | NA | NA | NO | NO | NT | |
| HLA-DRB1*15:02 | LYYQIHVLIGLALFAL | 0.3 | 0.9211 | 0.43948812 | NA | NA | NO | NO | NT | |
| HLA-DRB1*14:04 | LYESRLLRIASPMFHFGI | 0.38 | 0.7205 | 0.33801445 | NA | NA | NO | NO | NT | |
| HLA-DRB1*15:02 | MMGELFWTVVPYVTMTI | 0.39 | 0.7048 | 0.34250203 | NA | NA | NO | NO | NT | |
| Rv1737c | HLA-DQA1*06:01, DQB1*03:03 | AVTLASILPVLAV | 0.34 | 0.8199 | 0.38907504 | NA | NA | NO | NO | NT |
| HLA-DRB1*15:02 | ATMGSYALLVFFGLFL | 0.46 | 0.856 | 0.72004245 | NA | NA | NO | NO | NT | |
| Rv1980c | HLA-DRB3*02:02 | PAYNINISLPSYYPDQ | 0.08 | 1.3614 | 2 | NA | NA | NO | NO | NT |
| Rv1981c | HLA-DRB1*15:02 | FLFYSGFYLPMYW | 0.08 | 1.3283 | 1 | NA | NA | NO | NO | NT |
| HLA-DQA1*02:01, DQB1*05:02 | 0.12 | 1.3283 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DQA1*01:01, DQB1*05:01 | 0.16 | 1.3283 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DPA1*01, DPB1*04:01 | 0.19 | 1.3283 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DRB1*15:02 | SFLFYSGFYLPMYW | 0.06 | 1.072 | 1 | NA | NA | NO | NO | NT | |
| HLA-DPA1*01, DPB1*04:01 | 0.14 | 1.072 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DQA1*02:01, DQB1*05:02 | 0.16 | 1.072 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DQA1*01:01, DQB1*05:01 | 0.19 | 1.072 | 1 | NA | NA | NO | NO | NT | ||
| HLA-DPA1*01:03, DPB1*02:01 | 0.31 | 1.072 | 1 | NA | NA | NO | NO | NT | ||
| Rv2659c | HLA-DQA1*05:01, DQB1*03:01 | DLRVHDLRHSGAVLAAST | 0.44 | 0.9292 | 2 | NA | NA | NO | NO | NT |
| Rv3429 | HLA-DQA1*02:01, DQB1*05:02 | QSTARFILAYLPRWQ | 0.28 | 0.7639 | 0.13606656 | NA | NA | NO | NO | NT |
| HLA-DRB3*02:02 | AAAEQLRLMYNSANMTAK | 0.41 | 0.7112 | 0.54792199 | NA | NA | NO | NO | NT | |
| Rv3873 | HLA-DQA1*03:01, DQB1*06:01 | VAPSVMPAAAAGSSAT | 0.31 | 1.0173 | 0.23735279 | NA | NA | NO | NO | NT |
| HLA-DQA1*03:01, DQB1*06:01 | GLSAAAAKLAGLVF | 0.43 | 0.7126 | 0.67278691 | NA | NA | NO | NO | NT | |
| HLA-DQA1*05:01, DQB1*03:01 | DTGAGARPAASPLAAPV | 0.15 | 0.8414 | 0.82589324 | NA | NA | NO | NO | NT | |
| Rv3879 | HLA-DQA1*02:01, DQB1*05:02 | TGREAAHLRAFRAYAAHS | 0.03 | 0.7317 | 1.4848492 | NA | NA | NO | NO | NT |
| HLA-DQA1*05:01, DQB1*03:01 | VTPAAASGVPGARAAAAA | 0.47 | 0.8376 | 1.1311669 | NA | NA | NO | NO | NT | |
| HLA-DQA1*02:01, DQB1*05:02 | YPVLAVQAWAAFHDMTLR | 0.44 | 0.7948 | 0.84318227 | NA | NA | NO | NO | NT | |
| CTL | ||||||||||
| Rv1736c | HLA-B*15:02, HLA-B*15:11 | ELFWTVVPY | NA | 1.3999 | NA | 0.36591 | NA | NO | NO | NT |
| Rv1737c | HLA-A*02:01 | GVAGTIFAV | NA | 0.9924 | NA | 0.33154 | NA | NO | NO | NT |
| Rv2031c | HLA-A*33:03 | EMKEGRYEVR | NA | 1.678 | NA | 0.21929 | NA | NO | NO | NT |
| Rv2626c | HLA-A*33:03 | NVMEEHQVRR | NA | 0.8767 | NA | 0.12879 | NA | NO | NO | NT |
| Rv2656c | HLA-C*06:02, HLA-C*07:02 | RRDAYIRRV | NA | 0.9325 | NA | 0.24217 | NA | NO | NO | NT |
| Rv2659c | HLA-A*11:01 | ASYTGPDGR | NA | 1.0059 | NA | 0.09894 | NA | NO | NO | NT |
| Rv1511 | HLA-C*07:02, HLA-C*06:02 | LRPTEVDSL | NA | 1.2094 | NA | 0.09388 | NA | NO | NO | NT |
| Rv1980c | HLA-B*15:01 | GGTHPTTTY | NA | 1.7475 | NA | 0.12633 | NA | NO | NO | NT |
| Rv3872 | HLA-B*15:11 | EVSAQAATAF | NA | 0.8759 | NA | 0.00342 | NA | NO | NO | NT |
| Rv3425 | HLA-A*33:03 | EIAANREER | NA | 1.0439 | NA | 0.23749 | NA | NO | NO | NT |
| Rv3878 | HLA-A*33:03 | ETAAELAPR | NA | 1.0877 | NA | 0.16567 | NA | NO | NO | NT |
| Rv3879 | HLA-C*03:04, HLA-C*01:02, HLA-A*30:01 | ATHGANVSL | NA | 1.8216 | NA | 0.01479 | NA | NO | NO | NT |
| B cell | ||||||||||
| Rv1511 | NA | LIRRASTFNTSRIDHLYVDPHQPGARLFLHYGD | NA | NA | NA | NA | 0.77 | NO | NO | NT |
| Rv1737c | NA | LRDAPYFRPNADPVLPRLKAAA | NA | NA | NA | NA | 0.78 | NO | NO | NT |
| Rv1981c | NA | TLCSTKQIDDAFDWSEQNPYLQRKAQIIVDYY | NA | NA | NA | NA | 0.81 | NO | NO | NT |
| Rv2031c | NA | LEDEMKEGRYEVRAELPGVDPDKDV | NA | NA | NA | NA | 0.94 | NO | NO | NT |
| Rv2660c | NA | GVGTEQRNLSVVAPSQFTFSSRSPDFVDETAGQSWCAIL | NA | NA | NA | NA | 0.86 | NO | NO | NT |
| Rv2653c | NA | AGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEA | NA | NA | NA | NA | 0.92 | NO | NO | NT |
| Rv3425 | NA | GLANAYNDTRRKVVPPEEIAANREERRR | NA | NA | NA | NA | 0.86 | NO | NO | NT |
| Rv3879 | NA | TDQRLLDLLPPAPVDVNPPGDERHMLWFELMK | NA | NA | NA | NA | 0.82 | NO | NO | NT |
* AllerTOP v.2.0 and Allergen FP v.1.0 were used to predict allergenicity. NA: not applicable; NT: non-toxin; NO: non-allergenic.