TABLE 1.
Pseudomonas isolates and their characteristics
| Straina | Biolog ID (similarity) | Species ID by API 20 NEb | Enzyme activity for:
|
||
|---|---|---|---|---|---|
| Protease | Lipase | Lecithinase | |||
| Cluster A | |||||
| B1-054 | P. putida A (0.645) | P. putida | − | − | Brown |
| P. putida B (0.530) | |||||
| R1-057 | P. putida B1 (0.640) | P. putida | − | − | Brown |
| P. putida B1 (0.620) | |||||
| D1-034 | Deleya aesta (0.140) | P. fluorescens2 | + | + | − |
| Alcaligenes faecalis (0.351) | |||||
| D. marina (0.252) | |||||
| D. marina (0.611) | |||||
| B1-041 | P. putida A1 (0.815) | P. putida | − | − | − |
| B1-043 | P. putida A1 (0.811) | P. putida | − | − | − |
| R1-250 | P. putida A1 (0.835) | P. putida | − | − | − |
| P. putida A1 (0.834) | |||||
| D2-160 | P. putida A1 (0.891) | P. putida | − | − | − |
| D2-182 | P. putida A1 (0.501) | P. fluorescens | − | − | − |
| P. putida A1 (0.785) | |||||
| D1-082 | P. fulva (0.084) | P. putida | − | − | − |
| P. putida A1 (0.235) | |||||
| Cluster B1 | |||||
| D1-041 | P. corrugata (0.459) | P. fluorescens | + | +/− | + |
| P. tolasii (0.294) | |||||
| D1-043 | P. fluorescens A (0.374) | P. fluorescens10 | − | − | + |
| D1-077 | P. fluorescens A (0.635) | P. fluorescens | + | − | + |
| R1-146 | P. fluorescens A (0.796) | P. fluorescens | + | + | + |
| R1-051 | P. fluorescens A (0.632) | P. fluorescens1 | + | + | + |
| B1-069 | P. fluorescens A (0.793) | P. fluorescens1 | + | + | + |
| P. fluorescens A (0.866) | |||||
| R1-196 | P. fluorescens A (0.460) | P. fluorescens2 | + | + | + |
| R1-041 | P. fluorescens A (0.632) | P. fluorescens1 | + | + | + |
| R1-225 | P. fluorescens A (0.688) | P. fluorescens1 | + | + | + |
| R1-232 | P. fluorescens A (0.492) | P. fluorescens1 | + | + | + |
| R1-195 | P. tolasii (0.344) | P. fluorescens1 | + | + | + |
| R1-193 | P. tolasii (0.308) | P. fluorescens1 | + | + | + |
| P. tolasii (0.637) | |||||
| B1-052 | P. fluorescens A (0.977) | P. fluorescens1 | + | + | + |
| B1-045 | P. fluorescens A (0.552) | P. fluorescens1 | + | + | + |
| B1-048 | P. fluorescens A (0.552) | P. fluorescens1 | + | + | + |
| B1-047 | P. corrugata (0.301) | P. fluorescens1 | + | + | + |
| P. synxantha (0.653) | |||||
| B1-021c | P. fluorescens A (0.790) | P. fluorescens1 | + | + | + |
| D1-044 | P. corrugata (0.290) | P. fluorescens1 | + | +/− | + |
| P. corrugata (0.460) | |||||
| B1-044 | P. fluorescens A (0.760) | P. fluorescens1 | + | + | + |
| D2-059 | P. fluorescens A (0.748) | P. fluorescens | + | +/− | + |
| D1-019 | P. fluorescens A (0.875) | P. fluorescens1 | + | + | + |
| D1-025 | P. fluorescens A (0.647) | P. fluorescens7 | − | − | + |
| P. fluorescens A (0.526) | |||||
| D2-001 | P. fluorescens A (0.877) | P. fluorescens | + | +/− | + |
| P. fluorescens A (0.833) | |||||
| D2-004 | P. fluorescens A (0.661) | P. fluorescens | + | +/− | + |
| D1-040 | P. fluorescens A (0.806) | P. fluorescens | − | +/− | + |
| Cluster B2 | |||||
| B1-062 (B3) | P. fluorescens G (0.409) | P. fluorescens1 | + | + | − |
| P. fluorescens F (0.706) | |||||
| D1-028 | P. fluorescens A (0.801) | P. cepacia8 | + | + | + |
| D1-015 | P. fluorescens C (0.680) | P. pseudomallei6 | + | + | − |
| P. fluorescens C (0.794) | |||||
| D1-016 | P. fluorescens C (0.848) | P. pseudomallei6 | + | + | + |
| D1-048 | P. fluorescens C (0.760) | P. pseudomallei6 | + | + | + |
| P. fluorescens C (0.897) | (weak) | ||||
| D2-027 | P. fluorescens C (0.598) | P. fluorescens | − | − | − |
| P. fluorescens C (0.652) | |||||
| D2-048 | P. fluorescens C (0.729) | P. aeruginosa | + | +/− | + |
| B1-066 (B1) | P. fluorescens A (0.858) | P. fluorescens1 | + | + | + |
| B1-018d (B1) | P. fluorescens A (0.439) | P. fluorescens1 | − | − | − |
| P. tolasii (0.143) | |||||
| Cluster B3 | |||||
| B1-020d (B2) | P. fuscovaginae (0.702) | P. fluorescens | − | + | − |
| P. fuscovaginae (0.592) | |||||
| D1-045 | P. fluorescens B (0.467) | P. fluorescens1 | + | + | + |
| P. fluorescens G (0.609) | |||||
| B1-065 | P. fluorescens B (0.531) | P. fluorescens1 | + | + | + |
| P. fuscovaginae (0.610) | |||||
| B1-019e | P. marginalis (0.624) | P. fluorescens2 | + | + | − |
| P. corrugata (0.616) | |||||
| Cluster C | |||||
| B1-040 | P. fragi (0.794) | P. fluorescens3 | − | − | − |
| D1-081 | P. fragi (0.771) | P. putida | − | − | − |
| D1-018 | P. fragi (0.775) | P. putida | − | − | − |
| D1-014 | P. fragi (0.711) | P. fluorescens9 | − | + | − |
| P. fragi (0.635) | |||||
| D1-046 | P. fragi (0.833) | P. fluorescens9 | − | − | − |
| Cluster D | |||||
| D1-026 | P. pseudoalcaligenes (0.327) | P. putida | − | − | − |
| P. pseudoalcaligenes (0.329) | |||||
| D1-024 | P. fragi (0.450) | P. putida4 | − | − | − |
| P. cichorii (0.168) | |||||
| B1-033 | P. fragi (0.637) | P. putida4 | − | − | − |
| P. fulva (0.266) | |||||
| B1-060 | D. marina (0.513) | P. putida4 | − | − | − |
| D. marina (0.619) | |||||
| D1-027 | P. fulva (0.472) | P. putida4 | − | − | − |
| P. viridilivida (0.467) | |||||
| D2-329 | P. fragi (0.637) | P. putida | − | − | − |
| P. fragi (0.284) | |||||
| B1-032 | D. marina (0.415) | P. putida | − | − | − |
| P. fragi (0.168) | |||||
| B1-035 | D. marina (0.541) | P. putida | − | − | − |
| Cluster E | |||||
| B1-057 (A) | P. fragi (0.769) | P. putida | − | − | − |
| P. fragi (0.265) | |||||
| D2-017 | Acinetobacter genospecies 15 (0.417) | P. fluorescens | + | + | − |
| Acinetobacter genospecies 15 (0.438) | |||||
| D2-021 | Acinetobacter (0.445) | P. fluorescens | + | + | − |
| P. fragi (0.718) | |||||
| D2-036 | Psychrobacter immobilis (0.271) | P. fluorescens | + | + | − |
| Acinetobacter (0.556) | |||||
| D1-017 | P. fragi (0.418) | P. fluorescens | + | − | − |
| Acinetobacter genospecies 15 (0.389) | |||||
| D1-035 | Acinetobacter genospecies 15 (0.522) | P. fluorescens | + | + | − |
| P. mucidolens (0.346) | |||||
| D2-013 | P. fragi (0.799) | P. fluorescens | + | ++/− | − |
| B1-050 | Acinetobacter genospecies 15 (0.578) | P. putida5 | + | + | − |
| Acinetobacter genospecies 15 (0.580) | |||||
| D1-022 | Acinetobacter genospecies 15 (0.473) | P. putida5 | − | − | − |
| P. fulva (0.173) | |||||
| D1-021 | P. fragi (0.586) | P. putida5 | − | − | − |
| Acinetobacter genospecies 15 (0.602) | |||||
a
Each term in parentheses indicates the strain's resulting cluster designation when the phylogenetic analysis was repeated after eliminating substrate utilization data from the nine most variable Biolog substrates (see Results).
b
Species assigned by best likelihood; other possibilities indicated by superscript numbers as follows: 1, P. aureofaciens; 2, P. aureofaciens, P. chlororaphis; 3, P. putida; 4, P. fluorescens; 5, P. fluorescens, P. chlororaphis; 6, P. aeruginosa; 7, P. chlororaphis, P. aureofaciens; 8, P. aureofaciens, P. fluorescens; 9, P. putida, P. chlororaphis; 10, P. fluorescens, P. aureofaciens, P. cepacia.
c
Mushroom isolate.
d
Potato isolate.
e
Apple isolate.