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. 2023 May 1;133(9):e160451. doi: 10.1172/JCI160451

Figure 7. IGFBP7 induces endothelial glycocalyx degradation and promotes T cell adhesion.

Figure 7

(A and B) Representative staining and MFIs of HA and HS in HMEC-1 cells (n = 6/group) treated with PBS, endothelial glycocalyx degradation, or rhIGFBP7. (C and D) 3D reconstruction and quantification of HA and HS volume (n = 6/group). (E and F) Representative images and quantification of adherent CD4+ T cells after coculturing with HMEC-1 cells, which were pretreated with PBS, endothelial glycocalyx degradation, or rhIGFBP7 (n = 6/group). (G) Snapshots of CD4+ T cells flowing on HMEC-1 cells at different time points (n = 6/group). (H) The rolling velocity of CD4+ T cells in G (n = 20 T cells/group). (I) The number of adherent CD4+ T cells (n = 9 views/group) at the end of the flow assay in G. Data are represented as mean ± SD. All data were analyzed using 1-way ANOVA with Tukey’s post hoc test. Labeled P values in B and D represent the differences between the corresponding group and the PBS group.