FIG. 3.

DD comparing cells of A. fundyense in three different cell cycle stages (G₁, S, and G₂) and while STX production was turned on or off (I through IV refer to the four sampling points in Fig. 1; double lanes represent duplicate reverse transcription-PCRs). Band A is downregulated, while bands B and C are upregulated, relative to toxin production. Band D is constitutively expressed, while band E is G₁ specific (expressed in early and late G₁).