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. 2000 May;66(5):2113–2116. doi: 10.1128/aem.66.5.2113-2116.2000

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FIG. 1 — Multiplex PCR assay for the specific identification of L. pontis, L. panis, and the Lactobacillus sp. Primers indicated at the top are the specific primers for the identification of the following strains: L. pontis (LapoR) ATCC 51518 (lane 1), ATCC 51519 (lane 2), LTH 2587 (lane 3), and LTH 3572 (lane 4); L. panis (LapanR) DSM 6035 (lane 5) and DSM 6036 (lane 6); and Lactobacillus sp. strains (LaspecR) TMW 1.1098 (lane 7), TMW 1.1104 (lane 8), DSM 13145 (lane 9), and TMW 1.655 (lane 10). The specifically amplified fragment has a size of 236 bp (616V plus the specific reverse primer). The 800-bp fragment was amplified by the two universal primers 616V and 609R. Lane M, 100-bp ladder.