Table 4.
Summary of LC-MS/MS parameters and method performance parameters for all mycotoxins used.
| Compound | RT (min) | Precursor Ion (m/z) | Product Ion (m/z) |
Collision Energy | Polarity | % RA (RSD) | %SSE (RSD) | LOQ Oat |
|---|---|---|---|---|---|---|---|---|
| T-2 | 10.3 | 489.1 | 327.2 | −26.0 | +ve | 109.4 (2.2) | 103.3 (8.4) | 3.1 |
| HT-2 | 9.8 | 447.3 | 345.2 | −20.0 | +ve | 92.3 (6.8) | 91.4 (9.1) | 6.3 |
| T-2-Glc | 9.9 | 651.3 | 489.2 | −34.0 | +ve | 118.7 (3.6) | 96.5 (3.3) | 12.5 |
| HT-2-Glc | 9.4 | 609.2 | 447.1 | −34.0 | +ve | 114.1 (5.7) | 77.4 (8.8) | 12.5 |
| DAS | 8.9 | 384.2 | 307.5 | −12.0 | +ve | 95.3 (16.3) | 116.5 (5.0) | 25 |
| DAS-Glc | 8.5 | 551.2 | 389.1 | −33.0 | +ve | 112.0 (7.1) | 111.7 (1.8) | 25 |
| DON | 6.1 | 355.3 | 295.2 | 12.0 | −ve | 87.9 (7.6) | 107.3 (4.9) | 25 |
| DON-Glc | 5.9 | 517.2 | 427.2 | 23.0 | −ve | 95.1 (10.6) | 93.2 (6.4) | 12.5 |
| NIV | 5.3 | 371.2 | 281.2 | 20.0 | −ve | 101.1 (7.3) | 93.3 (7.4) | 12.5 |
| NIV-Glc | 5.1 | 533.3 | 473.2 | 14.0 | −ve | 94.7 (14.5) | 91.9 (2.8) | 12.5 |
| ZEN | 10.9 | 317.2 | 175.3 | 24.0 | −ve | 86.4 (6.4) | 104.4 (7.9) | 6.3 |
| ZEN-Glc | 9.4 | 479.4 | 317.2 | 21.0 | −ve | 70.4 (8.1) | 59.6 (5.0) | 6.3 |
| 13C22-HT-2 | 9.8 | 464.3 | 278.2 | −20.0 | +ve | |||
| 13C15-DON | 6.1 | 370.2 | 310.3 | 11.0 | −ve | |||
| 13C18-ZEN | 10.9 | 335.2 | 185.2 | 26.0 | −ve |