ABP	activity-based probe
ABPP	activity-based protein profiling
ADME	absorption, distribution, metabolism, and excretion
ALPHA	amplified luminescent proximity homogeneous assay
AOMK	acyloxymethyl ketone
APT	attached proton test
BME	β-mercaptoethanol
BODIPY	fluorinated boron-dipyrromethene
CAA	chloroacetamide
CITe-Id	covalent inhibitor target-site identification
CoLDR	covalent ligand directed release
CRI	covalent reversible inhibitor
cryo-EM	cryogenic electron microscopy
CuAAC	Huisgen copper-catalyzed alkyne–azide cycloaddition
DNP	2,4-dinitrophenyl
DPS	2,2′-dipyridyldisulfide
DTT	dithiothreitol
DUB	deubiquitinating enzyme
ELISA	enzyme-linked immunosorbent assay
FBDD	fragment-based drug development
FBLD	fragment-based ligand discovery
FDA	food and drug administration (USA)
FFPE	formalin fixed paraffin embedded
G12C-TE	KRASG12C target engagement
GSH	glutathione
GST	glutathione S-transferase
HCV	hepatitis C virus
His-	polyhistidine tag
HTS	high-throughput screening
IAc	iodoacetamide
ICC	intrahepatic cholangiocarcinoma
IEDDA	inverse electron demand Diels Alder
isoDTB	isotopically-labeled desthiobiotin
isoTOP-ABPP	isotopic tandem orthogonal activity-based protein profiling
iTRAQ	isobaric tag for relative and absolute quantitation
KD	dissociation constant
Ki*	steady-state inhibition constant
LC	liquid chromatography
MMTS	S-methyl methanethiosulfonate
MS	mass spectrometry
NMR	nuclear magnetic resonance
NSCLC	non-small cell lung carcinoma
ODN	odanacatib
PD	pharmacodynamics
PDB	protein data bank
PDT	photodynamic therapy
PET	photo-induced electron transfer
PK	pharmacokinetics
PMK	phenoxymethyl ketone
Prg	propargyl
PROTAC	proteolysis-targeting chimera
qABP	quenched ABP
Rho	rhodamine
SAR	structure-activity relationship
SARS-CoV-2	severe acute respiratory syndrome coronavirus 2
SDS-PAGE	sodium dodecyl sulfate polyacrylamide gel electrophoresis
SILAC	stable-isotope labeling by amino acids in cell culture
SPAAC	strain-promoted alkyne–azide cycloaddition
SUMO	small ubiquitin-related modifier
TAMRA/TMR	tetramethylrhodamine
TCEP	tris(2-carboxyethyl)phosphine
TCI	targeted covalent inhibitor
TIC	total ion count
THF	tetrahydrofuran
THL	tetrahydrolipstatin
TMB	tetramethyl benzidine
TMT	tandem mass tag
Ub	ubiquitin
VME	vinyl methyl ester
XRD	X-ray diffraction