TABLE 2.
Enzyme activity in cell extracts of S. paucimobilis SYK-6, its mutants, and recombinant strains
| Strain | Enzyme activitya (% of activity in SYK-6)
|
|||||
|---|---|---|---|---|---|---|
| DDVA O demethylation (LigX) | OH-DDVA dioxygenase (LigZ) | O demethylation (LigH) of:
|
Protocatechuate 4,5-dioxygenase (LigAB) acting on:
|
|||
| Syringate | Vanillate | 3-O-Methylgallate | Protocatechuate | |||
| SYK-6 | 100 | 100 | 100 | 100 | 100 | 100 |
| NT-1 | ND | 95 ± 5 | 93 ± 5 | 98 ± 5 | 99 ± 2 | 98 ± 3 |
| NT-11 | ND | ND | 90 ± 2 | 92 ± 3 | 98 ± 2 | 98 ± 4 |
| NT-21 | ND | ND | 95 ± 7 | 94 ± 4 | 97 ± 2 | 96 ± 5 |
| NT-23 | ND | ND | 93 ± 5 | 98 ± 3 | 95 ± 5 | 98 ± 3 |
| DC-49 | 75 ± 6 | 90 ± 4 | ND | ND | 97 ± 5 | 99 ± 3 |
| NT-1/pLE6 | 99 ± 3 | 97 ± 5 | 94 ± 3 | 96 ± 4 | 95 ± 3 | 99 ± 3 |
| DC-49/pLE6 | 72 ± 4 | 91 ± 5 | ND | ND | 95 ± 3 | 95 ± 4 |
a
LigX, LigZ, and LigAB enzyme activities were detected by a galavanic cell electrode. LigH enzyme activity was detected by GC (28). ND, not detected.