Skip to main content
. 2023 Mar 29;11(4):889. doi: 10.3390/microorganisms11040889

Figure 2.

Figure 2

Rooted phylogenetic tree of Spanish B. anthracis and representatives of the A.Br.011/009 canonical single-nucleotide polymorphisms (canSNP) clade derived from chromosomal SNP analysis. A total of 1817 chromosomal SNPs were used to construct a maximum parsimony tree (bootstrap confidence values were generated from 500 permutations). (A) Overview tree to illustrate the general positions of Spanish strains within the A.Br.011/009 phylogeny. (B) Close-up of the diversity of new branch A.Br11/ESPc. (C) Close-up of branching after canSNP A.Br.159. To indicate the positions of additional branches, these are drawn as condensed down to a single representative (grey wedges with strain names in brackets). Branch names or relevant canSNP positions are set in black or brown bold characters, respectively. Previously identified Spanish strains and branches are colored in green and new strains and clades in blue. All other relevant isolate names and countries of origin are indicated at branch termini. The red and blue boxes in panel A indicate the sections shown in detail in panels B and C. The tree is rooted to the B. anthracis reference strain Ames ‘Ancestor’, which belongs to the A.Br.Ames clade. a identical chromosomal sequence to isolates 21/890-8; 21/890-9; 21/903-6; 21/905-13; 21/918-3; 21/926-9; 21/926-22; 21/940-19 and 21/955-9; b identical chromosomal sequence to isolates 21/891-2 and 21/1088-3.