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. 2023 Apr 26;43(17):3042–3060. doi: 10.1523/JNEUROSCI.2226-22.2023

Figure 2.

Figure 2.

10 Hz rMS in organotypic tissue cultures. A, B, Entorhino-hippocampal tissue cultures (DAPI nuclear stain, B) were stimulated with a 70 mm outer wing diameter figure-of-eight coil (Magstim). Filter inserts carrying 2-6 tissue cultures were placed in a Petri dish below the coil. The orientation within the electromagnetic field and distance to the coil was kept constant in all experiments (DAPI nuclear stain). Scale bar, 200 µm. C, Sham-stimulated cultures were not stimulated but otherwise treated equally. After stimulation, cultures were kept in the incubator for 2-4 h before further experimental procedures, such as patch-clamp recordings.