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. 2023 Apr 28;18(4):e0285136. doi: 10.1371/journal.pone.0285136

Fig 2. Effects of TGM2 overexpression on proliferation, migration, and invasion of MDCK cells.

Fig 2

(A). mRNA levels of TGM2-overexpressed cells were determined with real-time PCR. (B). Protein levels of TGM2-overexpressed cells determined by Western blotting. (C). Effects of TGM2 overexpression on proliferation ability of MDCK cells determined by cell counting; (D,E) Effects of TGM2 overexpression on plate clone rate of MDCK cells determined by plate clone formation assays. (F,G) The effects of TGM2 overexpression on the invasion ability of MDCK cells were determined using Transwell assays. (H) The effects of TGM2 overexpression on the migration ability of MDCK cells were determined by scratch tests. TGM2-overexpressed cells (TGM2-OE); Vector control cell (Con). The experimental data are expressed as "mean ± standard deviation". *P < 0.05 and **P < 0.01 indicate significant differences, and ***P < 0.001 indicates extremely significant differences.