Fig 3. Effects of TGM2 gene knockout on proliferation, migration, and invasion of MDCK cells.

(A). mRNA level of TGM2-KO cells determined by real-time PCR; (B). Protein level of TGM2-KO cells determined by Western blotting; (C). Effect of TGM2 knockout on proliferation ability of MDCK cells determined by cell counting; (D,E). Effects of TGM2 knockout on the plate clone rate of MDCK cells were determined by plate cloning experiments. (F,G). Effects of TGM2 knockout on the invasion ability of MDCK cells determined by Transwell assay; (H). Effects of TGM2 knockout on migration capacity of MDCK cells determined by scratch test. TGM2-knockout cells (TGM2-KO); Wild-type cell (WT). The experimental data are expressed as "mean ± standard deviation". *P < 0.05 and **P < 0.01 indicate significant differences, and ***P < 0.001 indicates extremely significant differences.