Fig 4. Effects of TGM2 on proliferation of influenza A (H1N1) virus.

(A). mRNA levels of viral NP and NS1 genes overexpressed by TGM2 were determined by real-time PCR. (B, C). The effect of TGM2 overexpression on viral NP protein was determined by Western blotting. (D). Effects of TGM2 overexpression on titer of influenza A H1N1 virus determined by TCID₅₀; (E). mRNA levels of NP and NS1 genes of TGM2 knockout virus were determined by real-time PCR. (F, G). Effect of TGM2 knockout on NP protein of the virus was determined by Western blotting. (H). Effect of TGM2 knockout on titer of influenza A (H1N1) virus measured by TCID₅₀. TGM2-overexpressed cells (TGM2-OE); Vector control cells (Con); TGM2-knockout cells (TGM2-KO); Wild-type cell (WT). The experimental data are expressed as "mean ± standard deviation". *P < 0.05 and **P < 0.01 indicate significant differences, and ***P < 0.001 indicates extremely significant differences.