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(A and B) Reaction of heat-treated extract from cells of 2,6-DNT-grown JS850 with 3M4NC (A) and heat-treated extract from cells of E. coli JM109(pDTG603) (B) which expresses a cloned 3-methylcatechol-2,3-dioxygenase gene. (C) Addition of fresh (not heat-treated) cell extract from cells of 2,6-DNT-grown JS850 to the reaction product shown by line B.
