Figure 1.

Cell wall analysis of the different Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa morphologies. In (A), cells were treated with 25 U of endoglycosidase H for 20 h at 37 °C and the released N-linked glycans were quantified by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). In (B), cells were treated overnight with 0.1 N of NaOH, and the trimmed O-linked glycans were saved and quantified by HPAEC-PAD. Cells were stained with 1 mg mL⁻¹ of wheat germ agglutinin-fluorescein isothiocyanate (panel (C)) or with 5 μg mL⁻¹ of IgG Fc-Dectin-1 chimera, and then with 1 μg mL⁻¹ of donkey anti-Fc IgG-fluorescein (panel (D)) to stain chitin and β-1,3-glucan, respectively. The fluorescence of 300 cells for each condition was calculated and the median fluorescence was estimated as previously. The 100 % fluorescence corresponds to that calculated in heat-killed cells. S. schenckii—Sporothrix schenckii; S. brasiliensis—Sporothrix brasiliensis; S. globosa—Sporothrix globosa. Results are the median ± standard deviation from three independent experiments performed in triplicate. * p < 0.05 when compared to other morphologies from the same species; † p < 0.05 when compared to the same morphology in the other two fungal species; ‡ p <0.05 when compared to the same morphology in S. brasiliensis.